2× multif seamless assembly mix (ABclonal Biotechnology)
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ABclonal Biotechnology
2× multif seamless assembly mix
2× Multif Seamless Assembly Mix, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2%C3%97+multif+seamless+assembly+mix/2x+multif+seamless+assembly+mix/pmc12271464-367-19-24
Average 90 stars, based on 1 article reviews
2× Multif Seamless Assembly Mix, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/2%C3%97+multif+seamless+assembly+mix/2x+multif+seamless+assembly+mix/pmc12271464-367-19-24
Average 90 stars, based on 1 article reviews
2× multif seamless assembly mix - by Bioz Stars,
2026-10
90/100 stars
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Construct:Article Title: De novo designed protein guiding targeted protein degradation Article Snippet: .. All plasmids were constructed using standard ligation cloning techniques from Takara Bio (Dalian, China) or assembled using the 2× Ligation:Article Title: De novo designed protein guiding targeted protein degradation Article Snippet: .. All plasmids were constructed using standard ligation cloning techniques from Takara Bio (Dalian, China) or assembled using the 2× Article Title: Augmentation of exopolysaccharide synthesis and its influence on biofunctional properties of polysaccharide in Sanghuangporus vaninii via targeted overexpression of Phosphoglucomutase. Article Snippet: Phosphoglucomutase (PGM) is considered an essential catalyst in polysaccharide biosynthesis, plays a pivotal role in the early stage of carbon metabolism and the synthesis of nucleotide sugars.. Polysaccharides are important active components of Sanghuangporus vaninii.. Based on the transcriptomic data, the successful cloning and re-annotation of the putative 1353 bp PGM took place, which encodes a protein of 49.45 kDa. Cloning:Article Title: De novo designed protein guiding targeted protein degradation Article Snippet: .. All plasmids were constructed using standard ligation cloning techniques from Takara Bio (Dalian, China) or assembled using the 2× Article Title: Targeted knockout and plasmid-based transfer of NRPS/PKS for improving lipopeptide iturin A synthesis. Article Snippet: The nonribosomal peptide synthetase (NRPS)/polyketide synthase (PKS) assembly lines are a large enzymatic machinery that facilitates the transfer and synthesis of intermediates between multimodular megasynthases through complex protein–protein interactions.. Although NRPS/PKS systems display a highly sophisticated biosynthetic pathway, a similar strategy for the holistic editing of these gene clusters has not been described.. In this study, practical gene-editing tools were developed for long gene clusters, allowing the efficient knockout of entire NRPS/PKS gene clusters. Amplification:Article Title: Augmentation of exopolysaccharide synthesis and its influence on biofunctional properties of polysaccharide in Sanghuangporus vaninii via targeted overexpression of Phosphoglucomutase. Article Snippet: Phosphoglucomutase (PGM) is considered an essential catalyst in polysaccharide biosynthesis, plays a pivotal role in the early stage of carbon metabolism and the synthesis of nucleotide sugars.. Polysaccharides are important active components of Sanghuangporus vaninii.. Based on the transcriptomic data, the successful cloning and re-annotation of the putative 1353 bp PGM took place, which encodes a protein of 49.45 kDa. Article Title: Combinatorial metabolic engineering of Escherichia coli to efficiently produce L-threonine from untreated cane molasses. Article Snippet: • The global transcription factor Fur promotes the production of L-threonine.. • THR-48 efficiently produces 154.20 g/L L-threonine using glucose.. • THR-50 uses cane molasses to produce 92.46 g/L L-threonine cost-effectively. Article Title: Engineering a mouse-adapted SADS-CoV and establishing a neonatal mouse model to study its infection Article Snippet: .. Using the cDNA of these two viruses as templates, the required fragments were amplified, cloned and inserted into the pUC57 vector via 2× Plasmid Preparation:Article Title: Augmentation of exopolysaccharide synthesis and its influence on biofunctional properties of polysaccharide in Sanghuangporus vaninii via targeted overexpression of Phosphoglucomutase. Article Snippet: Phosphoglucomutase (PGM) is considered an essential catalyst in polysaccharide biosynthesis, plays a pivotal role in the early stage of carbon metabolism and the synthesis of nucleotide sugars.. Polysaccharides are important active components of Sanghuangporus vaninii.. Based on the transcriptomic data, the successful cloning and re-annotation of the putative 1353 bp PGM took place, which encodes a protein of 49.45 kDa. Article Title: Engineering a mouse-adapted SADS-CoV and establishing a neonatal mouse model to study its infection Article Snippet: .. Using the cDNA of these two viruses as templates, the required fragments were amplified, cloned and inserted into the pUC57 vector via 2× Purification:Article Title: Augmentation of exopolysaccharide synthesis and its influence on biofunctional properties of polysaccharide in Sanghuangporus vaninii via targeted overexpression of Phosphoglucomutase. Article Snippet: Phosphoglucomutase (PGM) is considered an essential catalyst in polysaccharide biosynthesis, plays a pivotal role in the early stage of carbon metabolism and the synthesis of nucleotide sugars.. Polysaccharides are important active components of Sanghuangporus vaninii.. Based on the transcriptomic data, the successful cloning and re-annotation of the putative 1353 bp PGM took place, which encodes a protein of 49.45 kDa. DNA Purification:Article Title: Augmentation of exopolysaccharide synthesis and its influence on biofunctional properties of polysaccharide in Sanghuangporus vaninii via targeted overexpression of Phosphoglucomutase. Article Snippet: Phosphoglucomutase (PGM) is considered an essential catalyst in polysaccharide biosynthesis, plays a pivotal role in the early stage of carbon metabolism and the synthesis of nucleotide sugars.. Polysaccharides are important active components of Sanghuangporus vaninii.. Based on the transcriptomic data, the successful cloning and re-annotation of the putative 1353 bp PGM took place, which encodes a protein of 49.45 kDa. other:Article Title: Protocol for identification of sgRNA mutants using high-throughput screening technique and multiplex genome editing Article Snippet: 2× MultiF seamless assembly mix , Article Title: An Advanced Bacterial Single-cell RNA-seq Reveals Biofilm Heterogeneity Article Snippet: DOI: https://doi.org/10.7554/eLife.97543 13 of 23 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Sequence- based reagent ccdB- F This paper PCR primers CACA GCGT TCAG ATAG TTAT TT TGTT AAAT GTAT TAAC TCTA G AGCG ACGC CAGA CG Sequence- based reagent ccdB- R This paper PCR primers CTGT AAGT ACGA ACTT ATTG AT TCTG GACA TACG TAAA TTAC GCCC CGCC CTGC CAC Sequence- based reagent Down- F This paper PCR primers TTTA CGTA TGTC CAGA ATCA ATAA GTTC GTAC TTAC Sequence- based reagent Down- R This paper PCR primers ATCT TCGT CAAA GGAT TTTC TGCC C Sequence- based reagent UP2- R This paper PCR primers ATCT TTTC GTAT AAAC TCAG CATG TTAA TACA TTTA AC AAAA TAAC TATC TGAA Sequence- based reagent pdeI- G412S- F This paper PCR primers ATGC TGAG TTTA TACG AAAA GATA AAGA T Sequence- based reagent pdeI- G412S- R This paper PCR primers CTTA TTGA TTCT GGAC ATAC GT AAAC TACT CTTT TACT AATT TTCC ACT Sequence- based reagent Down2- F This paper PCR primers TTTA CGTA TGTC CAGA ATCA ATAA GTTC GTAC TTAC Commercial assay or kit KAPA HIFI hotStart ReadyMix PCR Kits KAPA Cat#2602 Commercial assay or kit VAHTS Universal DNA Library Prep Kit Vazyme Cat#NR603 Commercial assay or kit Bacteria RNA Extraction Kit Vazyme Cat#R403- 01 Commercial assay or kit Ribo- off rRNA Depletion Kit (Bacteria) Vazyme Cat#N407 Commercial assay or kit 2× Clone Assay:Article Title: Engineering a mouse-adapted SADS-CoV and establishing a neonatal mouse model to study its infection Article Snippet: .. Using the cDNA of these two viruses as templates, the required fragments were amplified, cloned and inserted into the pUC57 vector via 2× |